Funding and Grants
R24 OD 011120
Enhanced Development of the Xiphophorus Model System
Specific Aims are: (1) To maintain and enhance the Xiphophorus Genetic Stock Center and provide the research community with animals, resources, and databases from genetically managed lines. (2) To sequence and annotate the genomes of X. hellerii, X. couchianus, and X. montezumae. (3) To establish a sperm repository for XGSC stocks and to extend protocols developed in our past project period with other Xiphophorus species.
R15 CA 223964 (Lu)
Identification of a Tumor Suppressor from the Xiphophorus Genome
Y. Lu and R. Walter [Texas State University] M. Schartl [University of Wuerzburg], and W. Warren [University of Missouri] multiple Co-PI’s.
Specific aims include: (1) Genotype expressed genes in Xiphophorus benign pigment cell lesions and spontaneous melanoma tumors, and define candidate R(Diff) loci on newly re-sequenced and gap-closed Xiphophorus genome assemblies. (2) Reduce the size of the candidate R(Diff) locus on chromosome 5 by genotyping R(Diff) candidates in large numbers of BC1 hybrid progeny. (3) Evaluate the effectiveness of R(Diff) candidate gene(s) in the ability to regulate the xmrk oncogene in the Xiphophorus pigment lesion cells and the xmrk-Tg medaka model.