Clonal Rainbow Trout Lines at Washington State University
|Gary Thorgaard || ||Sandra Ristow |
|Department of Zoology || ||Department of Animal Sciences |
|Washington State University || ||Washington State University |
|Pullman WA 99164-4236 || ||Pullman WA 99164-6310 |
|Email: email@example.com || ||Email: firstname.lastname@example.org |
Clonal lines of rainbow trout have been developed at Washington State University using the chromosome set manipulation techniques of androgenesis and gynogenesis. Androgenesis involves irradiating eggs with gamma radiation before fertilization, followed by a heat or pressure shock treatment to block the first cleavage division. An advantage of androgenesis is that it allows lines to be recovered from cryopreserved sperm. The isogenicity of the lines has been confirmed using multilocus RFLP studies (3,7).
Rainbow trout are a major biomedical research animal for cancer, immunology and toxiciology research. They are also advantageous because of the large amount of research that has been carried out with them related to aquaculture and sport fisheries. Rainbow trout are native to the Pacific coast of North America and Siberia and significant native populations till exist throughout much of the species range
Specific attributes of the five lines:
- Young, W.P., P.A. Wheeler and G.H. Thorgaard, 1995. Asymmetry and variability of menstic characters and spotting in isogenic lines of rainbow trout. Aquaculture 137: 67-76.
- Ristow, S.S., L.D. Grabowski, P.A. Wheeler, D.J. Prieur and G.H. Thorgaard, 1995. Arlee line of rainbow trout (Oncorhynchus mykiss) exhibits a low level of nonspecific cytotoxic cell activity. Dev. Comp. Immunol. 19: 497-505.
- Young, W.P., P.A. Wheeler, R.D. Fields and G.H. Thorgaard, 1996. DNA fingerprinting confirms isogenicity of androgenetically-derived rainbow trout lines. J. Hered. 87: 77-81.
- Ristow,.S.S., M.M. de Avila, T.J. Baldwin, P.A. Wheeler and G. H. Thorgaard, 1996. Acceptance of skin grafts by isogenic rainbow trout (Oncorhynchus mykiss). Am. J. Vet. Res. 57: 1576-1579.
- Young, W.P., P.A. Wheeler, V.H. Coryell, P. Kelm and G. H. Thorgaard, 1998. A detailed linkage map of rainbow trout produced using doubled haploids. Genetics 148: 839-850.
- Ristow, S.S., L.D. Grabowski, C. Ostberg, B. Robison and G.H. Thorgaard, 1998. Development of long-term cell lines from homozygous clones of rainbow trout. J. Aquat. Anim. Health 10: 75-82.
- Robison, B.D., P.A. Wheeler and G.H. Thorgaard. Variation in development rate among clonal lines of rainbow trout (Oncorhynchus mykiss). Aquaculture (in press).
- Ristow, S.S., L.D. Grabowski, S.M. Thompson, G.W.Warr, S.L. Kaattari, J.M. de Avila and G.H. Thorgaard. Coding sequences of the MHC II B chain of homozygous rainbow trout ( Oncorhynchus mykiss ). Dev. Comp. Immunol. (in press).
- Brunelli, J.P. and G.H. Thorgaard. Sequence, expression and genetic mapping of a rainbow trout retinoblastoma cDNA. Gene: (in press).
- Ristow, S. Washington State University, personal communication.
- Robison, B. Washington State University, personal communication.
- Wheeler, P. Washington State University, personal communication.